ELISpot Flex: Bovine IL-5 (ALP)

ELISpot Flex: Bovine IL-5 (ALP)

3191-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT52B34
Detection mAbMT11B37, biotin
Enzyme conjugateStreptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting bovine IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Bovine IL-5 (ALP)
ApplicationELISpot
AnalyteIL-5
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IL-5.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Bovine PBMC were incubated for 40 h in the absence (200,000 cells/well) or presence (100,000 cells/well) of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot.
Datasheet/Protocol
SDS

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Tutorial, Published December 28, 2023

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Tutorial, Published November 24, 2022

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In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, TRF
Cell typeT cell, Th2, NK cell
Gene ID280825